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Anti SUMO-1 Rabbit polyclonal Antibody

Price$219.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number21236
ImmunogenMouse
Size100 μl
Technical InformationView technical data
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  • Summary

    SUMO-1 [21236]
    [100 µL]


    Catalog Number:  21236
    Gene Symbol:  SUMO1
    Description:   Anti SUMO-1 Rabbit polyclonal Antibody
    Background:   Small Ubiquitin-like Modifier or SUMO proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2 and -3 show a high degree of similarity to each other and are distinct from SUMO-1.
    Immunogen:   A synthetic peptide corresponding to residues near the C-terminal of human SUMO-1.
    Applications:   ELISA, WB, IHC, IP
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:100-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Rabbit
    Format:   Liquid
    Clonality:   Polyclonal
    Isotype:   
    Purity:   Purified from serum
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Detects SUMO-1 but not SUMO-2/3 from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Western blot:  
    Western blot analysis of over-expressed SUMO-1 proteins. HEK293 cells were transfected with SUMO-1 (lane 2) and SUMO-2 (lane 3) cDNAs and control empty vector (lane 1). Cell lysates were blotted with anti SUMO-1 rabbit polyclonal antibody (Cat. # 21236).
    Publications:
    1.  The SUMOylation of zinc-fingers and homeoboxes 1 (ZHX1) by Ubc9 regulates its stability and transcriptional repression activity
        Journal of Cellular Biochemistry Volume 114, Issue 10, pages 2323–2333, October 2013
    2.  C-terminal Src Kinase (Csk)-mediated Phosphorylation of Eukaryotic Elongation Factor 2 (eEF2) Promotes Proteolytic Cleavage and Nuclear Translocation of eEF2
        J Biol Chem. 2014 May 2;289(18):12666-78