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Anti ATM Rabbit Polyclonal Antibody

Price$219.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number21276
ImmunogenMouse
Size100 μl
Technical InformationView technical data
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  • Summary

    ATM [21276]
    [100 µL]


    Catalog Number:  21276
    Gene Symbol:  ATM
    Description:   Anti ATM Rabbit Polyclonal Antibody
    Background:   Ataxia telangiectasia mutated (ATM) is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. It phosphorylates several key proteins that initiate activation of the DNA damage checkpoint, leading to cell cycle arrest, DNA repair or apoptosis. Activity of ATM protein is under tight control, and mutation of ATM can cause disease such as Ataxia telangiectasia (AT) and cancers.
    Immunogen:   Purified ATM protein, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:100-1:1000 
    IHC: 1:50-1:200
    Concentration:    0.2mg/ml
    Host:   Rabbit
    Format:   Liquid
    Clonality:   Polyclonal
    Isotype:   
    Purity:   Purified from serum
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes ATM of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Western blot:  
    Western blot analysis of recombinant ATM protein. Purified His-tagged ATM protein was blotted with Anti ATM rabbit polyclonal antibody (Cat. #21276).
    Immunohistochemistry:  
    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Anti ATM polyclonal antibody (Cat. #21276). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.


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